Methylene blue-fast green staining of hemopoietic colonies in agar cultures

A simple technique is described for the routine in situ identification of the cellular composition of colonies and clusters in agar cultures of hemopoietic cells. The entire culture, dried and formalin vapor fixed within a Petri dish, is stained with a mixture of methylene blue and fast green. By th...

Full description

Saved in:

Bibliographic Details
Published in:	Gegenbaurs morphologisches Jahrbuch Vol. 135; no. 5; p. 779
Main Authors:	Zvetkova, E B, Jelinek, J
Format:	Journal Article
Language:	English
Published:	Germany 1989
Subjects:	Basophils - ultrastructure Cell Nucleus - ultrastructure Cells, Cultured Cytoplasmic Granules - ultrastructure DNA - analysis Eosinophils - ultrastructure Hematopoietic Stem Cells - analysis Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - ultrastructure Histocytochemistry Humans Macrophages - ultrastructure Methylene Blue Monocytes - ultrastructure Neutrophils - ultrastructure Proteins - analysis RNA - analysis Rosaniline Dyes Staining and Labeling
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A simple technique is described for the routine in situ identification of the cellular composition of colonies and clusters in agar cultures of hemopoietic cells. The entire culture, dried and formalin vapor fixed within a Petri dish, is stained with a mixture of methylene blue and fast green. By this method cellular ribonucleoproteins (RNP), deoxyribonucleoproteins (DNP) and some cationic (arginine and lysine containing) proteins are detected. Different maturation stages of neutrophils, eosinophils, basophils, monocytes, and macrophages can be easily identified with colonies and clusters on the basis of the cytoplasmic and nuclear staining.
ISSN:	0016-5840