Translocation of protein kinase C α and ζ in rat glomerular mesangial cells cultured under high glucose conditions

• Published in: Diabetologia Vol. 37; no. 8; pp. 838 - 841
• Main Authors: KIKKAWA, R, HANEDA, M, UZU, T, KOYA, D, SUGIMOTO, T, SHIGETA, Y
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-08-1994
• Subjects: Animals; Associated diseases and complications; Biological and medical sciences; Cell Membrane - enzymology; Cells, Cultured; Cytosol - enzymology; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Gene Expression; Glomerular Mesangium - drug effects; Glomerular Mesangium - enzymology; Glucose - pharmacology; Isoenzymes - isolation & purification; Isoenzymes - metabolism; Kinetics; Male; Medical sciences; Protein Kinase C - isolation & purification; Protein Kinase C - metabolism; Rats; Rats, Sprague-Dawley; Time Factors; Chromosomal aberration; Endocrinopathy; Immunopathology; Cell culture; Protein kinase C; Urinary system disease; Rat; Enzyme; Mesangial cell; Isozyme; Rodentia; Autoimmune disease; Renal disease; Renal glomerulus; Vertebrata; Mammalia; Nephropathy; Enzymatic activity; Animal; Insulin dependent diabetes; Abnormal chromosome; Chromosome translocation
• ISSN: 0012-186X; 1432-0428
• DOI: 10.1007/BF00404342

The activities and expression of protein kinase C isoenzymes were examined in glomerular mesangial cells cultured under high glucose conditions. Exposure of cells to high glucose concentrations (27.8 mmol/l) for more than 3 days resulted in a significant elevation of protein kinase C activities in the membrane fraction. Of the protein kinase C isoenzymes, the levels of protein kinase C alpha significantly increased in the membrane fraction after 3 days of exposure to glucose, and protein kinase C zeta increased after 5 days of exposure. Levels of protein kinase C delta and epsilon remained unchanged and protein kinase, C beta and gamma were not detected. These results indicate that protein kinase C alpha and zeta are translocated under high glucose conditions possibly through different mechanisms.