Wild-Type Isocitrate Dehydrogenase 1 Over-Expression is Related to Cancer Stem Cells Survival in Lung Adenocarcinoma

• Published in: Cancer investigation Vol. 36; no. 3; pp. 185 - 189
• Main Authors: Al-Amodi, Hiba Saeed A Bagader, Nabih, Enas Samir, Kamel, Hala Fawzy M, El Sayed, Mohamed Ali, Dwedar, Ibrahim Aly Mohamed
• Format: Journal Article
• Language: English
• Published: England 16-03-2018
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma of Lung; Aged; Cell Survival; Female; Gene Expression Regulation, Neoplastic; Glutathione - metabolism; Humans; Hyaluronan Receptors - genetics; Isocitrate Dehydrogenase - genetics; Lung Neoplasms - genetics; Male; Middle Aged; Neoplastic Stem Cells - cytology; Up-Regulation; adenocarcinoma; metabolism; GSH; CD44; CSCs
• ISSN: 1532-4192
• DOI: 10.1080/07357907.2018.1445262

Altered metabolism is one of the characteristics of cancer cells. We evaluated the expression of wild-type Isocitrate Dehydrogenase 1 (IDH1) and the cancer stem cells (CSCs) marker CD44 by real-time PCR and levels of reduced form of glutathione in lung biopsies of 32 adenocarcinoma patients and 18 control subjects. We found that IDH1 and CD44 expression and the levels of reduced form of glutathione were significantly higher in lung adenocarcinoma patients. IDH1 was positively correlated with CD44 and reduced form of glutathione. In conclusion, wild-type IDH1 is over-expressed in lung adenocarcinoma which probably promoted tumor progression via increasing CSCs survival.