Cytokine-regulating activity of anti-virus preparation CelAgripus in Burkitt's lymphoma stable B-cell lines.

• Published in: Voprosy virusologii Vol. 64; no. 4; p. 165
• Main Authors: Narovlyansky, A N, Mezentseva, M V, Suetina, I A, Russu, L I, Ivanova, A M, Poloskov, V V, Izmest'eva, A V, Ospelnikova, T P, Sarymsakov, A A, Ershov, F I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2019
• Subjects: Antiviral Agents - pharmacology; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Burkitt Lymphoma - drug therapy; Burkitt Lymphoma - immunology; Burkitt Lymphoma - virology; Cell Line, Tumor; Cytokines - genetics; Gene Expression Regulation, Neoplastic - drug effects; Herpesvirus 4, Human - genetics; Herpesvirus 4, Human - pathogenicity; Humans; Immunity, Innate - drug effects; Immunity, Innate - genetics; Interferon-alpha - genetics; Interferons - genetics; Tumor Necrosis Factor-alpha - genetics; Virus Diseases - genetics; Virus Diseases - virology; Burkitt’s lymphoma; interferon inducer; the polymerase chain reaction; Epstein-Barr virus; cytokines; gene expression; stable cell lines
• ISSN: 2411-2097
• DOI: 10.36233/0507-4088-2019-64-4-165-172

Cytokines activated in response to immunosuppressive viral infections can directly or indirectly affect the neoplastic transformation of B cells. In this study, we studied a new substance designed to produce the antiviral drug CelAgrip (CA, CelAgripus), which exhibits interferon (IFN) and cytokine-inducing activity and, apparently, can be used as an activator of antiviral immunity. Purpose - is to evaluate the cytokine-regulating effect of CA in Burkitt's lymphoma (LB) cell lines latently infected with the Epstein-Barr virus (EBV).
to study the CA-induced expression of the cytokine genes IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, IL-18, IFN-α, IFN -γ, IFN-β, IFN-λ1, IFN-λ2, IFN-λ3, TNF-α in normal and EBV transformed LB cells.
Cell line: the human embryo fibroblasts (HEF), Namalva, Daudi, Raji, P3HR-1. Preparations: CA, gossypol-acetic acid (GAA), sodium carboxymethyl cellulose (Na-CMC).
RT-PCR and methods for assessing cytotoxicity (MTT and Scepter 2.0 Merck cell counter).
The effect of the CA preparation on the expression of IFN-λ, IL-1β, IL-6, IL-8 and IL-10 genes was revealed.
We observed the activation of gene expression of IFN-λ, IL-1β, IL-6, IL-8 and suppression of IL-10 gene activity when treatment CA of LB cells.
The substance CA has new effects on the activation of the expression of a number of key cytokine genes in stable Burkitt lymphoma cell lines.