Serum sialic acid, cellular anionic charge and erythrocyte aggregation in diabetic and hypertensive patients

• Published in: Medicina (Buenos Aires) Vol. 69; no. 3; pp. 331 - 334
• Main Authors: Lebensohn, Natalia, Re, Andrea, Carrera, Larisa, Barberena, Liliana, D'Arrigo, Mabel, Foresto, Patricia
• Format: Journal Article
• Language: Spanish
• Published: Argentina 2009
• Subjects: Adult; Aged; Anions - metabolism; Case-Control Studies; Diabetes Mellitus - blood; Erythrocyte Aggregation; Female; Humans; Hypertension - blood; Male; Middle Aged; N-Acetylneuraminic Acid - blood
• ISSN: 0025-7680

The aim of this work was to study the relationship between serum sialic acid (SSA) and erythrocyte anionic charge (EAC) with erythrocyte aggregation in two groups: diabetic (DBT, n=20) and hypertensive (HT, n=21) patients, compared to a control group (n=20). We worked with anticoagulated blood with EDTA and serum. The erythrocyte aggregation was studied by microscopically observing and quantifying aggregates using an ASP (Aggregate Shape Parameter). The EAC was determined by binding an Alcian blue dye to the membrane sialic acid and SSA was determined by spectrophotometric method with an Erlich reactant. The values of ASP and SSA increased significantly in HT and DBT patients compared to the control group. The HT and DBT groups showed amorphous aggregates, evident in an alteration in the values of ASP, which were significantly higher ( p < 0.005) than in healthy patients. The EAC values were much lower in HT and DBT patients than in the control group (p < 0.0001). In this work, abnormalities in the erythrocyte aggregation could be detected by the values of ASP, EAC and SSA, which might be involved in vascular disorders of diseases such as hypertension and diabetes.