Parallel confocal detection of single molecules in real time

The confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations. This is achieved through the use of a holographic laser illumination multiplexer combined with a confocal pinhole array before a prism dispersive elem...

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Bibliographic Details
Published in:	Optics letters Vol. 33; no. 9; p. 1026
Main Authors:	Lundquist, Paul M, Zhong, Cheng F, Zhao, Peiqian, Tomaney, Austin B, Peluso, Paul S, Dixon, John, Bettman, Brad, Lacroix, Yves, Kwo, Deborah P, McCullough, Etienne, Maxham, Mark, Hester, Kevin, McNitt, Paul, Grey, Donald M, Henriquez, Carlos, Foquet, Mathieu, Turner, Stephen W, Zaccarin, Denis
Format:	Journal Article
Language:	English
Published:	United States 01-05-2008
Subjects:	Fluorescent Dyes - chemistry Microscopy, Confocal - instrumentation Microscopy, Confocal - methods Nanotechnology
Online Access:	Get more information
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Summary:	The confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations. This is achieved through the use of a holographic laser illumination multiplexer combined with a confocal pinhole array before a prism dispersive element used to provide spectroscopic information from each confocal volume. The system is demonstrated to detect and identify single fluorescent molecules from each of several thousand independent confocal volumes in real time.
ISSN:	0146-9592
DOI:	10.1364/OL.33.001026