Expression of the AML-1 oncogene shortens the G(1) phase of the cell cycle

• Published in: The Journal of biological chemistry Vol. 275; no. 5; pp. 3438 - 3445
• Main Authors: Strom, D K, Nip, J, Westendorf, J J, Linggi, B, Lutterbach, B, Downing, J R, Lenny, N, Hiebert, S W
• Format: Journal Article
• Language: English
• Published: United States 04-02-2000
• Subjects: Animals; Cell Cycle - genetics; Cell Line; Core Binding Factor Alpha 2 Subunit; DNA-Binding Proteins; Flow Cytometry; G1 Phase - genetics; Gene Expression Regulation; Humans; Mice; Proto-Oncogene Proteins - genetics; Transcription Factors - genetics; Transfection
• ISSN: 0021-9258
• DOI: 10.1074/jbc.275.5.3438

The AML-1-encoded transcription factor, AML-1B, regulates numerous hematopoietic-specific genes. Inappropriate expression of AML-1-family proteins is oncogenic in cell culture systems and in mice. To understand the oncogenic functions of AML-1, we established cell lines expressing AML-1B to examine the role of AML-1 in the cell cycle. DNA content analysis and bromodeoxyuridine pulse-chase studies indicated that entry into the S phase of the cell cycle was accelerated by up to 4 h in AML-1B-expressing 32D.3 myeloid progenitor cells as compared with control cells or cells expressing E2F-1. However, AML-1B was not able to induce continued cell cycle progression in the absence of growth factors. The DNA binding and transactivation domains of AML-1B were required for altering the cell cycle. Thus, AML-1B is the first transcription factor that affects the timing of the mammalian cell cycle.