Enhanced nucleolar parameters in Vicia faba L. induced by 5-azacytidine may be only partly attributed to DNA demethylation

We aimed to find if diminishing rDNA expression in differentiating and differentiated root parenchyma, in spite of DNA endoreplication, could be related to rDNA methylation. The effect of 5-azacytidine (10(-6)M, 72 h) was studied in successive segments of Vicia faba roots. In 5-azacytidine-treated c...

Full description

Saved in:

Bibliographic Details
Published in:	Folia histochemica et cytobiologica Vol. 35; no. 3; p. 185
Main Authors:	Olszewska, M J, Sakowicz, T, Kaźmierczak, J, Luchniak, P
Format:	Journal Article
Language:	English
Published:	Poland 1997
Subjects:	Azacitidine - pharmacology Blotting, Southern Cell Nucleolus - drug effects Cell Nucleolus - genetics Chromatography, High Pressure Liquid DNA Methylation - drug effects DNA Replication - drug effects DNA, Plant - biosynthesis DNA, Plant - chemistry Fabaceae - cytology Fabaceae - drug effects Fabaceae - genetics Models, Biological Plants, Medicinal Transcription, Genetic - drug effects
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We aimed to find if diminishing rDNA expression in differentiating and differentiated root parenchyma, in spite of DNA endoreplication, could be related to rDNA methylation. The effect of 5-azacytidine (10(-6)M, 72 h) was studied in successive segments of Vicia faba roots. In 5-azacytidine-treated cells, nucleolar parameters: [3H] uridine incorporation, size, amount of AgNOR protein and the number of bi-nucleolate cells were increased. [3H] uridine incorporation and the amount of AgNOR protein were significantly correlated. In meristems, HPLC analysis demonstrated a decrease in m5dC content by 3.7%. Southern hybridization showed demethylation at CCGG sites in repetitive BamHI sequences and discrete demethylation in coding region of rDNA.
ISSN:	0239-8508