Effects of caspase-1 inhibitor VX765 on cold-restraint stress-induced acute gastric ulcer in mice

• Published in: Zhonghua shao shang za zhi Vol. 33; no. 11; p. 688
• Main Authors: Zheng, S Q, Hong, X D, Chen, T S, Luo, P F, Xiao, S C
• Format: Journal Article
• Language: Chinese
• Published: China 20-11-2017
• Subjects: Animals; Blotting, Western; Burns - physiopathology; Enzyme-Linked Immunosorbent Assay; Interleukin-18; Interleukin-1beta; Male; Mice; Mice, Inbred C57BL; Serpins - metabolism; Serpins - pharmacology; Stomach Ulcer - drug therapy; Tumor Necrosis Factor-alpha; Viral Proteins - metabolism; Viral Proteins - pharmacology; VX765; Stomach ulcer; Cold-restraint stress; Caspase 1
• ISSN: 1009-2587
• DOI: 10.3760/cma.j.issn.1009-2587.2017.11.006

To investigate the protective effects of caspase-1 inhibitor VX765 on gastric mucosa of mice with cold-restraint stress-induced acute gastric ulcer.
Twenty-four specific pathogen free male C57BL/6J mice were divided into normal control group (NC), cold restrain group (CR), VX765 pre-treatment+ cold restrain group (VCR), and rabeprazole pre-treatment+ cold restrain group (RCR) according to the random number table, with 6 mice in each group. Mice in group NC were injected intraperitoneally with solution of 10 mL/kg dimethylsulfoxide (DMSO) and phosphate buffer solution (PBS). Mice in group CR were inflicted with acute gastric ulcer induced by cold-restraint stress 30 minutes after intraperitoneal injection of solution of DMSO and PBS. Mice in groups VCR and RCR were inflicted with acute gastric ulcer as above 30 minutes after intraperitoneal injection of solution of DMSO and PBS with dose of 12.5 μmol/kg containing 10 mg VX765 and 40 mg/kg containing 20 mg rabeprazole, respectively. Four hour after cold-restrai