Synthesis of peptide fragments of high-affinity receptor FcepsilonR1 and study their binding with allergen-specific immunoglobulin E

• Published in: Bioorganicheskaia khimiia Vol. 38; no. 3; p. 291
• Main Authors: Gribovskaia, O V, Martinovich, V P, Golubovich, V P, Ianchenko, V V, Vykhristenko, L R, Novikov, D K
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-05-2012
• Subjects: Allergens - immunology; Antigens, Dermatophagoides - immunology; Asthma - immunology; Binding Sites; Chemistry Techniques, Synthetic; Humans; Hypersensitivity; Immunoglobulin E - immunology; Immunoglobulin Fc Fragments - chemistry; Immunoglobulin Fc Fragments - immunology; Peptide Fragments - chemical synthesis; Peptide Fragments - chemistry; Peptide Fragments - immunology; Protein Binding; Receptors, IgE - chemistry; Receptors, IgE - immunology
• ISSN: 0132-3423

High-affinity receptor FcepsilonR1 is a key substance which participates in IgE-dependent allergic reactions of immediate type. A minimal sequence Arg136-Asn137-Trp138-Asp139, which takes part in binding with C3 and C4 fragments of IgE was determined by methods of computer analysis. As possible analogs of FcepsilonR1 receptor, capable of binding to Fc-fragment IgE, a number of peptide compounds containing this sequence were proposed. Biological researches have shown that these peptides possess immunobiological effect and bind to IgE. Studied the ability of peptides to bind with IgE class serum antibodies specific to allergen Dermatophagoides pteronyssinus in patients with allergic bronchial asthma.