The effect of ubiquitin ligase Cullin3 on the proliferation, migration and invasion of triple-negative breast cancer cells and exploration of its mechanism

• Published in: Zhong hua yi xue za zhi Vol. 104; no. 20; p. 1868
• Main Authors: He, Z X, Li, J, Wang, Q F, Cheng, Q, Ni, S J
• Format: Journal Article
• Language: Chinese
• Published: China 28-05-2024
• Subjects: Cell Line, Tumor; Cell Movement; Cell Proliferation; Cullin Proteins - metabolism; Female; Humans; Neoplasm Invasiveness; Prognosis; Triple Negative Breast Neoplasms - metabolism; Triple Negative Breast Neoplasms - pathology; Ubiquitination
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.cn12137-20240110-00071

To investigate the effects of ubiquitin ligase Cullin3 (CUL3) on the proliferation, migration and invasion ability of triple-negative breast cancer (TNBC) cells and its mechanism of action.
Bioinformatics-based methods were used to obtain CUL3 gene and protein expression data in TNBC tissues, and to assess the expression of CUL3 in tumour tissues of TNBC patients (
=160) and in normal breast tissues (
=572), and its relationship with clinical prognosis. The effects of overexpression of CUL3 on the proliferation, migration and invasion ability of TNBC cells in vitro were detected by CCK8 cell proliferation assay, scratch assay and transwell assay; proteins that might interact with CUL3 were screened by immunoprecipitation combined with mass spectrometry analysis, and the substrate protein regulated by CUL3 was identified as Glutathione S-Transferase Pi 1 (GSTP1); the effects of overexpression of GSTP1 on the migration and invasion ability of TNBC cells were detected by scratch assay and Transwell assay, and it