Regulation of the genes expression of CD4+ T-lymphocytes differentiation transcription factors by galectin-3 in vitro

• Published in: Molekuliarnaia biologiia Vol. 47; no. 6; p. 1004
• Main Authors: Vasil'eva, O A, Iakushina, V D, Riazantseva, N V, Novitskiĭ, V V, Tashireva, L A, Starikova, E G, Zima, A P, Prokhorenko, T S, Krasnova, T Iu, Nebesnaia, I S
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-11-2013
• Subjects: Adult; CD4-Positive T-Lymphocytes - drug effects; CD4-Positive T-Lymphocytes - immunology; Cell Differentiation - drug effects; Female; Galectin 3 - administration & dosage; Galectin 3 - genetics; Galectin 3 - metabolism; GATA3 Transcription Factor - biosynthesis; Gene Expression Regulation - drug effects; Gene Expression Regulation - immunology; Humans; Male; Nuclear Receptor Subfamily 1, Group F, Member 3 - biosynthesis
• ISSN: 0026-8984
• DOI: 10.7868/s0026898413060165

Now a number of CD4+ T-lymphocytes, known as Th1, Th2, Treg and Th17, is currently identified and well- studied. The methods basing on the targeted regulation of differentiation process of the Th-lymphocytes that carry out the immune response polarization attract an attention of scientists dealing with a correction of immune-mediated. In the present study, endogenous beta-galactoside-binding protein of the lectin family, galectin-3, was investigated as a regulator of T-cell homeostasis. A galectin-3 is known to be actively produced by tumor cells in malignant transformation and able to influence the processes of signal transduction, cell-cell cooperation and the implementation of programmed death. As cell differentiation processes are directly connected with the regulation of gene expression, we investigated the effect of recombinant galectin-3 on expression of mRNA of transcription.factors, which guide the differentiation of CD4+ lymphocytes. The study was performed on peripheral blood mononuclear cells of healthy individuals. The gene expression levels were evaluated by a real-time PCR. In the experiments in vitro, it has been first found the recombinant galectin-3 (0.5 mg/mL) up-regulating the expression of transcription factors Gata-3 and Rorc mRNAs and down-regulating the mRNA expression of transcription factors T-bet and FoxP3. Up to a concentration of 1 mg/mL recombinant galectin-3 stimulates Th-cells by dose-dependent manner, whereas at higher concentrations stimulating effect weakens, and inhibiting action starts prevailing. Thus, one can suppose that galectin-3 through regulation of lymphocytes differentiation promote development of allergic, autoimmune and neoplastic diseases that allows us to consider the galectin-3 as a.potential target for therapy of these diseases.