The Effect of the Chromophore Charge on the Efficiency of Incorporation of Fluorescently-labeled Nucleotides in Matrix Synthesis by Taq DNA Polymerase

In order to study the effect of an electrical charge of the chromophore, on the efficiency of incorporation of fluorescently-labeled nucleotides into DNA during PCR, three fluorescently-labeled dUPT, one of which with electroneutral and other two with positively and negatively charged dyes (Cy5 anal...

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Bibliographic Details
Published in:	Biofizika Vol. 60; no. 6; p. 1216
Main Authors:	Shershov, V E, Kuznetsova, V E, Lysov, Yu P, Guseinov, T O, Barsky, V E, Spitsyn, M A, Zasedateleva, O A, Vasiliskov, V A, Surzhikov, S A, Zasedatelev, A S, Chudinov, A V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-11-2015
Subjects:	DNA - chemistry DNA - isolation & purification Fluorescent Dyes - chemistry Nucleotides - chemistry Polymerase Chain Reaction - methods Taq Polymerase - chemistry
Online Access:	Get more information
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Summary:	In order to study the effect of an electrical charge of the chromophore, on the efficiency of incorporation of fluorescently-labeled nucleotides into DNA during PCR, three fluorescently-labeled dUPT, one of which with electroneutral and other two with positively and negatively charged dyes (Cy5 analogs), were synthesized. It is shown that dUPT, labeled with electroneutral Cy 5 analog, is most effectively incorporated into DNA when Tag polymerase is used for PCR.
ISSN:	0006-3029