Effect of circBIRC6 targeting miR-367-3p on cisplatin resistance of ovarian cancer cells

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 43; no. 10; p. 1062
• Main Authors: Zhang, J, Bai, H J
• Format: Journal Article
• Language: Chinese
• Published: China 23-10-2021
• Subjects: Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Apoptosis; Cell Line, Tumor; Cell Proliferation; Cisplatin - pharmacology; Cisplatin - therapeutic use; Drug Resistance, Neoplasm - genetics; Female; Humans; Inhibitor of Apoptosis Proteins; MicroRNAs - genetics; Ovarian Neoplasms - drug therapy; Ovarian Neoplasms - genetics; RNA, Circular - genetics; Ovarian neoplasms; CircBIRC6; Proliferation; Drug resistance; Cisplatin; Apoptosis; Micro-367-3p
• ISSN: 0253-3766
• DOI: 10.3760/cma.j.cn112152-20200427-00384

To explore the effect of circBIRC6 on cisplatin resistance of ovarian cancer cells and the molecular mechanism.
The ovarian cancer cell line SKOV3 and ovarian cancer cisplatin-resistant cell line SKOV3 / DDP were cultured in vitro, and treated with different concentrations of cisplatin. SKOV3 and SKOV3/DDP cells were transfected with si-NC, si-circBIRC6, si-circBIRC6+ anti-miR-NC, si-circBIRC6+ anti-miR-367-3p by liposome-mediated method, which were denoted as DDP+ si-NC group, DDP+ si-circBIRC6 group, DDP+ si-circBIRC6+ anti-miR-NC group and DDP+ si-circBIRC6+ anti-miR-367-3p group, respectively, and then were treated with 2 μg/ml cisplatin for 24 hours. The cell proliferation inhibition rate was detected by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide method, and the half inhibitory concentration (IC(50)) value of cisplatin was calculated. Real-time fluorescent quantitative polymerase chain reaction was used to detect the transcriptional levels of circBIRC6 and miR-367-3p. Flow cytomet