Immunohistochemical assay of murine liver tissue using monoclonal antibodies to NO-synthase 2 and TNF-α under tetrachloromethane toxic injury conditions

• Published in: Ėksperimentalʹnaâ i kliničeskaâ farmacologiâ Vol. 78; no. 2; p. 20
• Main Authors: Pechenkina, I G, Kozin, S V, Bulanov, D V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2015
• Subjects: Animals; Antibodies, Monoclonal - chemistry; Carbon Tetrachloride Poisoning - drug therapy; Carbon Tetrachloride Poisoning - metabolism; Carbon Tetrachloride Poisoning - pathology; Eleutherococcus - chemistry; Gene Expression - drug effects; Immunohistochemistry; Liver - drug effects; Liver - metabolism; Liver - pathology; Male; Mice; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - metabolism; Phytotherapy; Plant Extracts - pharmacology; Protective Agents - pharmacology; Silymarin - pharmacology; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0869-2092

Experimental data showing a significant role of enzyme NO-synthase type 2 and cytokine TNF-α enzymes in pathogenesis of CCl4-induced liver injury have been obtained by immunohistochemical assay. It is established that the hepatotoxic agent leads to an increase in NO-synthase 2 and TNF-α levels. The use of both studied phytoadaptogens (eleutherococcus extract and ginseng tincture) at a doses of 48.5 mg/kg and 47 mg/kg, respectively, and the reference drug karsil (100 mg/kg daily for 5 weeks) prevented to a significant degree the accumulation of enzyme NO-synthase 2 and the cytokine TNF-α, which is apparently one of the mechanisms of hepatoprotective action.