The identification of clinical strains Pseudomonas fulva using techniques of MALDI-TOF mass spectrometry and common analysis

The technique MALDI-TOF mass spectrometry was applied using device Microflex with database MALDI Biotyper (Bruckeer Daltonics Inc.) to identify with high level of reliability 8 strains P. fulva from collection of pseudo monads isolated from clinical material in St. Petersburg. When analyzing the sam...

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Bibliographic Details
Published in:	Klinicheskaia laboratornaia diagnostika Vol. 60; no. 1; p. 46
Main Authors:	Sivolodskiĭ, E P, Zueva, E B, Kunilova, E S, Bogumil'chik, E A, Domakova, T V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-01-2015
Subjects:	Bacteriological Techniques - methods Humans Oxidoreductases - biosynthesis Oxidoreductases - genetics Pigments, Biological - biosynthesis Pigments, Biological - genetics Pseudomonas Infections - diagnosis Pseudomonas Infections - genetics Pseudomonas Infections - microbiology Pseudomonas putida - genetics Pseudomonas putida - isolation & purification Pseudomonas putida - pathogenicity Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:	The technique MALDI-TOF mass spectrometry was applied using device Microflex with database MALDI Biotyper (Bruckeer Daltonics Inc.) to identify with high level of reliability 8 strains P. fulva from collection of pseudo monads isolated from clinical material in St. Petersburg. When analyzing the same strains applying technique MALDI TOF mass spectrometry using device Vitek MS (bioMerieux) these starins were wrongly identifies as P. putida. The complex of tests of common analysis was approved and proposed for control differentiation of P. fulva and P. putida. The medical significance of P. fulva was approved.
ISSN:	0869-2084