ENHANCEMENT OF AGROBACTERIAL TRANSFORMATION OF PLANTS USING PROTEIN KINASE INHIBITORS TRIFLUOPERAZINE AND GENISTEIN

The effect of different concentrations of protein tyrosine kinase inhibitor, genistein and serine/threonine protein kinase inhibitor, trifluoperazine, on the frequency of Agrobacterium-mediated transformation of leaf explants of N. tabacum was investigated. The influence of different concentrations...

Full description

Saved in:

Bibliographic Details
Published in:	TSitologiia i genetika Vol. 50; no. 1; p. 3
Main Authors:	Yemets, A I, Fedorchuk, V V, Blume, Ya B
Format:	Journal Article
Language:	Ukrainian
Published:	Ukraine 01-01-2016
Subjects:	Agrobacterium tumefaciens - drug effects Agrobacterium tumefaciens - genetics Dose-Response Relationship, Drug Genistein - pharmacology Nicotiana - drug effects Nicotiana - enzymology Nicotiana - genetics Nicotiana - growth & development Plant Leaves - drug effects Plant Leaves - enzymology Plant Leaves - genetics Plant Leaves - growth & development Protein Kinase Inhibitors - pharmacology Transformation, Genetic - drug effects Trifluoperazine - pharmacology
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The effect of different concentrations of protein tyrosine kinase inhibitor, genistein and serine/threonine protein kinase inhibitor, trifluoperazine, on the frequency of Agrobacterium-mediated transformation of leaf explants of N. tabacum was investigated. The influence of different concentrations of trifluoperazine in the range from 10 to 300 μM was investigated. It was found that 10 μM trifluoperazine provoked the increase of the frequency of agrobacterial transformation of tobacco leaf disks on 25%. In parallel, the influence of different concentrations of genistein in the range from 10 to 100 μM was investigated. It was found 100 μM genistein provoked the increase of the frequency of agrobacterial transformation of tobacco leaf disks on 12%.
ISSN:	0564-3783