Effect of tandem repeats adjacent to 3'-terminal of FLO1 on the flocculation function of Saccharomyces cerevisiae

• Published in: Wei sheng wu hsüeh pao Vol. 53; no. 12; p. 1276
• Main Authors: Yue, Feng, Du, Zhaoli, Guo, Xuena, He, Xiuping, Zhang, Borun
• Format: Journal Article
• Language: Chinese
• Published: China 04-12-2013
• Subjects: Amino Acid Motifs; Animals; Flocculation; Hydrogen-Ion Concentration; Mannose - metabolism; Mannose-Binding Lectins - chemistry; Mannose-Binding Lectins - genetics; Mannose-Binding Lectins - metabolism; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Tandem Repeat Sequences; Temperature
• ISSN: 0001-6209

Many tandem repeats exist in FLO1 gene of Saccharomyces cerevisiae, which might have great regulatory effect on the conformation and function of flocculation protein (flocculin). In this study, we analyzed the effect of 3'-terminal tandem repeats B, C and D complete deletion on the function of flocculin.
We constructed the derived gene FLO1 bcd with complete deletion of tandem repeats B, C and D of FLO1 by fusion PCR. We then constructed plasmid pYCF1 bcd by insertion of FLO1 bcd into YCp50, and transformed such plasmid, pYCF1 and YCp50 into S. cerevisiae YS58 separately to generate recombinant strains YSF1 bcd, YSF1 and YSP50. We compared the flocculation ability and characteristics of these strains.
Compared to YSF1, YSF1 bcd displayed only a slight reduction (4%) in flocculation ability in optimal flocculation buffer (50 mmol/L NaAC, pH 4.5). Moreover, the dependence of flocculation on Ca2+, sensitivity to metal ions and ethanol, and the specificity to different sugars showed no obvious difference between