Zbtb33 Gene Knockout Changes Transcription of the Fgf9, Fgfr3, с-Мус and FoxG1 Genes in the Developing Mouse Brain

• Published in: Molekuliarnaia biologiia Vol. 55; no. 3; p. 422
• Main Authors: Illarionova, N B, Borisova, M A, Bazhenova, E Y, Zabelina, D S, Fursenko, D V, Kulikov, A V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-05-2021
• Subjects: Animals; Brain; Fibroblast Growth Factor 9; Forkhead Transcription Factors - genetics; Gene Knockout Techniques; Mice; Mice, Inbred C57BL; Mice, Knockout; Nerve Tissue Proteins; Proto-Oncogene Proteins c-myc; Transcription Factors - genetics; Wnt/β-catenin signaling; Kaiso; mouse; frontal cortex; Real-time PCR; hippocampus; striatum; Fgf9; c-Myc; FoxG1; Fgfr3
• ISSN: 0026-8984
• DOI: 10.31857/S0026898421030095

The transcription factor KAISO is important for proper development of animal embryos. In the cell, KAISO regulates cell division and apoptosis. KAISO is abundant in the central nervous system. Here we describe the effects of Zbtb33 gene knockout on the transcription of several genes that regulate the development of the central nervous system, including Fgf9, Fgfr3, Sox9, Sox2, c-Myc, NeuroD1 and FoxG1. These genes are related to the Wnt/β-catenin signaling pathway, which is closely connected to KAISO. Hippocampal, frontal cortical, and striatal tissue from C57BL/6j mice with a knockout in the Zbtb33 gene encoding KAISO (ZBTB33-) and wild-type mice (ZBTB33+) were collected and profiled at different stages of development. Age-dependent and region-specific differences in the mRNA levels of the Fgf9, Fgfr3, c-Myc, FoxG1 genes in the developing brain of ZBTB33- and ZBTB33+ mice were described and discussed.