The inhibitory effect of CtBP-interacting protein on the oxidative damage of cerebral endothelia cells

• Published in: Zhong hua yi xue za zhi Vol. 100; no. 14; p. 1102
• Main Authors: Du, G J, Geng, Dangmurenjiafu, Fan, Y D, Su, R Q, Bate, Gonggaoang
• Format: Journal Article
• Language: Chinese
• Published: China 14-04-2020
• Subjects: Cell Line; Endothelial Cells; Oxidative Stress; Inhibit; CtIP gene; Cerebral endothelia cells; Oxidative damage
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.cn112137-20190919-02074

To explore the effect of CtBP-Interacting protein (CtIP) on oxidative damage of cerebral endothelia cells and its mechanism.
Cerebral endothelia cells were stimulated by TBHP to induce oxidative damage. The cell line of CtIP gene were prepared by over-expression and interfering lentivirus technology. Cell damage was detected by immunofluorescence assay of cysteinyl aspartate specific proteinase-3 (Caspase-3). The expression of CtIP and Caspase-3 protein was detected by Western blotting, and the related genes of CtIP signaling pathway were detected by Realtime RT-PCR.
The results of immunofluorescence and Western blotting showed that overexpression of CtIP inhibited the Caspase-3 expression reducing to 1/3 level compared with normal cultured cerebral endothelia cells. Interfering with CtIP expression resulted in the Caspase-3 expression increased significantly to 4/5 level compared with normal cultured cerebral endothelia cells and cerebral endothelia cells were damaged more severely. Realtime RT-PCR data show