Effects of PKCα/c-fos, Bax/Bcl-2 expression on the proliferation and apoptosis of rat pulmonary arterial smooth muscle cells in hypoxia

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 33; no. 3; p. 218
• Main Authors: Cao, Yan-Hong, Pan, Jian-Xun, Liang, Yue-Qin, Shi, Rui-Zan
• Format: Journal Article
• Language: Chinese
• Published: China 08-03-2017
• Subjects: Animals; Apoptosis; bcl-2-Associated X Protein - metabolism; Cell Hypoxia; Cell Proliferation; Cells, Cultured; Muscle, Smooth, Vascular - cytology; Myocytes, Smooth Muscle - cytology; Protein Kinase C-alpha - metabolism; Proto-Oncogene Proteins c-bcl-2 - metabolism; Proto-Oncogene Proteins c-fos - metabolism; Pulmonary Artery - cytology; Rats; hypoxia; apoptosis; proliferation; Bax/Bcl-2; PKCα/c-fos
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.5442.2017.054

To explore the effects of PKCα/c-fos, Bax/Bcl-2 on the proliferation and apoptosis of rat pulmonary arterial smooth musclecells(PASMCs) in hypoxia.
The PASMCs of rats had been isolated and cultured, and then were cultured under normoxia (5%CO
、21%O
、74%N
)and hypoxia(5%CO
,2% O
,93%N
)condition for 24 h and 48 h, respectively. The proliferation of PASMCs was tested by methylthiazolyltetrazolium (MTT). The changes of PASMCs apoptosis were detected by Annexin V-FITC/PI staining combined flow cytometry. RT-PCR and Western blot analysis were performed to detect the gene and protein levels of PKCα/c-fos, Bax/Bcl-2, respectively. All experiments were repeated three times with at least triplicate samples.
The proliferation of PASMCs in hypoxia group were stronger than that of normoxia group after 24 h and 48 h (
< 0.01), while the apoptosis rate did not change significantly. Meanwhile, the higher levels of PKCα, c-fos, Bcl-2 mRNA and proteins after hypoxia for 24 h and 48 h were detected by RT-PCR and Western blot a