Clinical application value of peripheral blood metagenomic next-generation sequencing test for patients with hematological diseases accompanied by fever

• Published in: Zhōnghuá xuèyèxué zázhì Vol. 43; no. 9; p. 766
• Main Authors: Hao, S F, Wang, Y H, Li, L J, Wang, H Q, Song, J, Wu, Y H, Qu, W, Wang, G J, Wang, X M, Liu, H, Xing, L M, Guan, J, Shao, Z H, Fu, R
• Format: Journal Article
• Language: Chinese
• Published: China 14-09-2022
• Subjects: Fever; Hematologic Diseases; Hematologic Tests; High-Throughput Nucleotide Sequencing; Humans; Retrospective Studies; Sensitivity and Specificity; Clinical adjudication; Hematological diseases; Metagenomic next generation sequencing
• ISSN: 0253-2727
• DOI: 10.3760/cma.j.issn.0253-2727.2022.09.009

To investigate the clinical application value of peripheral blood metagenomic next-generation sequencing (mNGS) test for patients with hematological diseases accompanied by fever.
The blood mNGS results and clinical data of inpatients with hematological diseases accompanied by fever treated in the Hematology Department of Tianjin Medical University General Hospital in March 2020 to June 2021were retrospectively analyzed. A total of 90 patients with 98 cases of specimens were included. The pathogen distribution characteristics and mNGS test performance were analyzed.
The positive rate of peripheral blood mNGS was significantly higher than that of traditional examination (68.37%
37.76%,
<0.001) and blood culture (68.37%
9.18%,
<0.001) . Viral, bacterial, and fungal infections accounted for 38.81%, 14.93%, and 2.99% in patients with single-pathogen infections, respectively. Polymicrobial infections accounted for 43.28%, in which viral and bacterial coinfections were the most common type (25.37%) . There were 55