Detection of BCR/ABL fusion gene by fluorescence in situ hybridization and its clinical application

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 19; no. 5; p. 1283
• Main Authors: Zhou, Rui-Lian, Mo, Yao-Xi, Lan, Mei, Lin, Jin-Ying
• Format: Journal Article
• Language: Chinese
• Published: China 01-10-2011
• Subjects: Adolescent; Adult; Aged; Child; Female; Fusion Proteins, bcr-abl - genetics; Genes, abl; Humans; In Situ Hybridization, Fluorescence - methods; Leukemia - diagnosis; Leukemia - genetics; Male; Middle Aged; Myeloproliferative Disorders - diagnosis; Myeloproliferative Disorders - genetics; Young Adult
• ISSN: 1009-2137

This study was aimed to investigate the clinical value of detecting BCR/ABL fusion gene by fluorescence in situ hybridization (FISH). The conventional cytogenetic test and detection of BCR/ABL fusion gene by FISH for bone marrow of patients with newly diagnosed chronic myeloproliferative disease or myelodysplastic and myeloproliferative disorders, acute lymphocytic leukemia and chronic myelogenous leukemia (CML) after allogeneic hematopoietic stem cell transplantation were carried out. The results showed that (1) out of 46 newly diagnosed as chronic myeloproliferative disease or myelodysplastic and myeloproliferative disorders, 22 cases were diagnosed as CML, the FISH detection showed all positive (100%), while cytogenetic test showed 86.4% (19/22) positive, in the other 24 patients who were diagnosed as other chronic myeloproliferative disease or myelodysplastic and myeloproliferative disorders, BCR/ABL fusion gene all were be detected as negative 100% by FISH, while the cytogenetic test of bone marrow in 3