The development and testing of a molecular biological test system for DNA detection of anthrax pathogen by real-time polymerase chain reaction assay

The paper presents the results of the development and testing of a molecular biological test system for DNA detection of anthrax pathogen (Bacillus anthracis) by real-time polymerase chain reaction assay. The test system has shown high sensitivity, specificity, and reproducibility of results of anal...

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Bibliographic Details
Published in:	Klinicheskaia laboratornaia diagnostika no. 3; p. 46
Main Authors:	Abramov, D D, Vorob'ev, A A, Kuznetsovskiĭ, A V, Savinykh, A V, Onuchina, N V, Darmov, I V, Trofimov, D Iu, Kuznetsov, S L
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-03-2011
Subjects:	Animals Anthrax - diagnosis Antigens, Bacterial - genetics Bacillus anthracis - genetics Bacillus anthracis - isolation & purification Bacterial Toxins - genetics Cricetinae DNA, Bacterial - analysis Humans Mesocricetus Polymerase Chain Reaction - methods Time Factors
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Summary:	The paper presents the results of the development and testing of a molecular biological test system for DNA detection of anthrax pathogen (Bacillus anthracis) by real-time polymerase chain reaction assay. The test system has shown high sensitivity, specificity, and reproducibility of results of analysis, as exemplified by aqueous suspensions of daily agar cultures of Bacillus anthracis strains, related and heterologous species of microorganisms, and clinical materials of experimental animals. There is evidence for the persistence of the basic characteristics of the test system when stored at 22 +/- 2 degrees C for 12 months.
ISSN:	0869-2084