Immune effects of four coxsackievirus B3 VP1 DNA fusion vaccines in mice

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 26; no. 2; p. 103
• Main Authors: Liu, Gui-xia, Lan, Jia-ming, Chuai, Xia, Gao, Zhi-yun, Jin, Yu-huai, Zhang, Yong-hong, Xie, Li-xin, Yin, Chang-fu, Wang, Yong-xiang
• Format: Journal Article
• Language: Chinese
• Published: China 01-02-2010
• Subjects: Animals; Antibodies, Viral - blood; beta-Defensins - genetics; Capsid Proteins - genetics; Capsid Proteins - immunology; Chemokine CCL22 - genetics; Complement C3d - genetics; Enterovirus B, Human - immunology; Male; Mice; Mice, Inbred BALB C; Recombinant Fusion Proteins - immunology; Shiga Toxin - genetics; Vaccination; Vaccines, DNA - immunology; Viral Vaccines - immunology
• ISSN: 1007-8738

To compare the immunogenicity and protective effects on CVB3 infected mice of four DNA fusion vaccines coupling coxsackievirus B3 (CVB3) VP1 with macrophage-derived chemokine (MDC), C3d3, shiga toxin B subuit (STxB) and mouse beta-defensin-2 (mBD2), respectively.
BALB/c mice were divided into 6 groups randomly and inoculated in quadriceps at 3-week interval for 3 times with pcDNA3, pcDNA3/VP1, pcDNA3/MDC-VP1, pcDNA3/VP1-C3d3, pcDNA3/STxB-VP1 and pcDNA3/mBD2 -VP1, respectively. Fourteen days after every inoculation, serum samples were collected and CVB3 specific neutralizing antibodies were determined. Three weeks after the last immunization, the mice were treated in three ways. First, the spleen cells were isolated from 3 mice of each group and specific CTL activities were tested. Second, 3 mice of each group were further challenged with 3LDLD(50); CVB3 and sacrificed 7 days later, and their blood viral titers were evaluated. Third, the rest mice of each group were subjected to intraperitoneal (i.p.) challeng