The effect of inhibitors of serinethreonine protein kinases on Arabidopsis thaliana root morphology and microtubules organization in its cells

• Published in: T͡S︡itologii͡a Vol. 52; no. 5; p. 389
• Main Authors: Sheremet, Ia A, Emets, A I, Vissenberg, K, Verbelen, J -P, Blium, Ia B
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2010
• Subjects: Arabidopsis - drug effects; Arabidopsis - growth & development; Arabidopsis - ultrastructure; Meristem - metabolism; Microtubules - drug effects; Microtubules - metabolism; Microtubules - ultrastructure; Phosphorylation; Plant Roots - drug effects; Plant Roots - growth & development; Plant Roots - ultrastructure; Protein Kinase Inhibitors - pharmacology; Protein-Serine-Threonine Kinases - antagonists & inhibitors
• ISSN: 0041-3771

The effect of different types of serine/thereonine protein kinases inhibitors (cyclin-dependent, Ca2+-calmodulin-dependent and protein kinase C) on microtubules organization in cells of Arabidopsis thaliana main primary root zones were investigated in vivo. The microtubules in epidermal and cortex cells in the transition and elongation zones as well as microtubules in trichoblasts and atrichoblasts in the differentiation zone showed the greatest sensitivity to protein kinases inhibitors studied. It was established that microtubules in these cell types modified their initial transverse/oblique orientation to a chaotic or longitudinal relative to the major axis of primary root as a result of serinethereonine protein kinases inhibition. The microtubules in cells in root meristematic zone as well as in root hairs were less sensitive to influence of protein kinases inhibitors tested. Alterations of microtubules orientation in the cells in primary root zones under the influence of serinethereonine protein kinases inhibitors led to further disturbances in growth and differentiation processes. It was assumed that phosphorylation of microtubules proteins, especially tubulin, might be involved in the regulation of these processes.