Transformation of full-length gene of mouse/human chimeric antibody against Hantaan virus into Arabidopsis thaliana

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 24; no. 1; p. 49
• Main Authors: Zhou, Wei, Wu, Xing-an, Luo, Wen, Hu, Gang, Zhang, Fang-lin, Bai, Wen-tao, Zhang, Liang, Yu, Lan, Shi, Meng-yuan, Xu, Zhi-kai
• Format: Journal Article
• Language: Chinese
• Published: China 01-01-2008
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antigens, Viral - immunology; Arabidopsis - genetics; Arabidopsis Proteins - immunology; Cloning, Molecular; Gene Expression Regulation, Plant; Genetic Vectors - genetics; Hantaan virus - genetics; Hantaan virus - immunology; Humans; Mice; Plants, Genetically Modified - growth & development; Plants, Genetically Modified - immunology; Polymerase Chain Reaction; Recombinant Proteins - genetics; Recombinant Proteins - immunology
• ISSN: 1007-8738

To construct the transgenic Arabidopsis thaliana containing full-length gene of mouse/human chimeric antibody(3G1MH) against Hantaan virus.
The recombinant plasmid 3G1MH-pCAMBIA2301 was transformed into Agrobacterium tumefaciens GV3101 by TSS freeze-thaw method, and then the recombinant was transferred into wild Arabidopsis thaliana by vacuum-transgenic method. The regenerated transgenic plants were selected with kanamycin, and confirmed by PCR and Northern blot.
PCR result showed stable integration of the 3G1MH gene IN Arabidopsis thaliana genome in 7 stains of the transformed plants. Northern blot analyses confirmed the transcription of heavy and light chains in the transgenic plants.
The successful establishment of 3G1MH transgenic Arabidopsis thaliana plants pave the way for further research on expressing therapeutic antibody in transgenic plants.