Study on multi-lineage potential of bone marrow mesenchymal stem cells derived from green fluorescent protein transgenic mice

• Published in: Hua xi kou qiang yi xue za zhi Vol. 23; no. 2; p. 152
• Main Authors: Li, Zhi-Yong, Tian, Wei-Dong, Liu, Lei, Chen, Xi-Zhe, Lin, Yun-Feng, Yan, Zheng-Bin, Chen, Ling, Li, Sheng-Wei
• Format: Journal Article
• Language: Chinese
• Published: China 01-04-2005
• Subjects: Alkaline Phosphatase - physiology; Animals; Bone Marrow Cells; Cell Differentiation; Cells, Cultured; Centrifugation, Density Gradient; Green Fluorescent Proteins - metabolism; Mesenchymal Stromal Cells; Mice; Mice, Transgenic; Neurons; Osteoblasts
• ISSN: 1000-1182

To study the multi-lineage potential of bone marrow mesenchymal stem cells (MSCs) derived from transgenic mice with green fluorescent protein (GFP) gene in vitro.
A 6-week-old GFP transgenic mouse was executed by dislocation of cervical vertebra, and the marrow in tibia and thighbone was washed out with asepsis. The limited cell strains of MSCs derived from GFP transgenic mice (GFP-MSCs) were obtained with density gradient centrifugation. The passage 3 GFP-MSCs were induced to differentiate into osteoblast, adippcyte, neuron with solution of calcium induction medium, adipogenic medium and neural induction medium respectively. After being calcium-induced, the activity of alkaline phosphatase on GFP-MSCs was determined by micro-plate reader, and alizarin red staining was performed to test the formation of calcium concentration. The adipo-induced MSCs were detected with oil red O staining. Immunocytochemical staining was performed to detect the expression of NSE on neuron-induced MSCs.
The ALP activity of GFP-MS