Comparative analysis of SGLT1 and GLUT2 transporter distribution in rat small intestine enterocytes and Caco2 cells during hexose absorption

• Published in: T͡S︡itologii͡a Vol. 52; no. 7; p. 580
• Main Authors: Grefner, N M, Gromova, L V, Gruzdkov, A A, Komissarchik, Ia Iu
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2010
• Subjects: Animals; Caco-2 Cells; Enterocytes - metabolism; Glucose Transporter Type 2 - metabolism; Hexoses - metabolism; Humans; Intestinal Absorption; Intestine, Small - cytology; Intestine, Small - metabolism; Male; Microscopy, Confocal; Microscopy, Electron; Rats; Rats, Wistar; Sodium-Glucose Transporter 1 - metabolism
• ISSN: 0041-3771

SGLT1 and GLUT2 hexose transporter distribution into enterocytes of small intestine isolated loop and Caco2 cell culture after absorption of high and low hexose concentrations has been considered. SGLT1 was found along intestine villus edge in isolated loop. After high concentration hexose load GLUT2 appeared to be situated in the apical parts of enterocytes. It is evident that GLUT2 participates in hexose transport across apical membrane. Cultured Caco2 cells form microvilli and cell junction complex typical for enterocyte. Glucose and galactose absorption by the cells from incubation medium has been observed. SGLT1 transporter is situated in the apical parts and around the nuclens of Caco2 cells and combined into globules. After low concentration hexose load, CLUT2 transporter is localized in the basal parts of Caco2 cells. Caco2 cell culture can be used as a model for studying of hexose transport in small intestine epithelium.