Establishment of subcutaneously transplanted and metastatic neuroblastoma models in nude mice

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 32; no. 4; p. 258
• Main Authors: Lu, Hong-ting, Dong, Qian, Gao, Qiang, Hao, Xi-wei, Song, Hua, Zhao, Nan
• Format: Journal Article
• Language: Chinese
• Published: China 01-04-2010
• Subjects: Animals; Cell Line, Tumor; Child, Preschool; Disease Models, Animal; Female; Gene Expression Profiling; Humans; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Liver Neoplasms - secondary; Male; Mice; Mice, Nude; Neoplasm Invasiveness; Neoplasm Metastasis; Neoplasm Transplantation; Neuroblastoma - genetics; Neuroblastoma - metabolism; Neuroblastoma - pathology; Neuroblastoma - secondary; Phosphopyruvate Hydratase - metabolism; Tumor Burden
• ISSN: 0253-3766

To establish a tumor-bearing nude mouse model of human neuroblastoma in order to study the mechanisms of neuroblastoma invasion and metastasis, and to investigate potential therapeutic modalities in the experimental animal models.
A human neuroblastoma cell line was cultured in vitro. 1 x 10(7) cells undergoing exponential growth were collected in 0.1 ml of suspension and subcutaneously inoculated into the right flank next to the forelimb in nude mice. The biological characteristics of the developed tumors were observed, and histopathological and DNA microarray analyses were performed. The expressions of NSE in the subcutaneous tumor, metastatic tumor and the primary neuroblastoma tumor tissues from a pediatric patient were analyzed by immunohistochemistry.
Tumors successfully grew in 36 out of 48 injected mice, with a total tumor-formation rate of 75.0%. Metastasis occurred in 10 cases, and the metastatic rate was 20.8%. Tumors in five injected mice grew locally without metastasis. These tumors had large vol