In vitro effect of cyclosporine A on juvenile Schistosoma mansoni labeled by AF18 (5-N-octadecanoyl aminofluorescein)

To explore the in vitro effect of cyclosporine A on the tegument of juvenile Schistosoma mansoni labeled by AF18 and investigate the effect of cyclosporine A on schistosomula surface membrane fluidity. Preparation of transformed schistosomula, adding cyclosporine A into tubes containing schistosomul...

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Bibliographic Details
Published in:	Zhongguo jishengchongxue yu jishengchongping zazhi Vol. 22; no. 6; p. 328
Main Authors:	Liu, Jian-fa, Kusel, John R
Format:	Journal Article
Language:	English
Published:	China 01-12-2004
Subjects:	Animals Cyclosporine - pharmacology Dose-Response Relationship, Drug Fluorescein - metabolism Membrane Fluidity - drug effects Microscopy, Fluorescence Schistosoma mansoni - drug effects Schistosoma mansoni - physiology
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Summary:	To explore the in vitro effect of cyclosporine A on the tegument of juvenile Schistosoma mansoni labeled by AF18 and investigate the effect of cyclosporine A on schistosomula surface membrane fluidity. Preparation of transformed schistosomula, adding cyclosporine A into tubes containing schistosomula and labeling of transformed schistosomula with AF18, then observe schistosomula under fluorescence microscope. Schistosomula of different groups labeled by AF18 were damaged by cyclosporine A in vitro. Cyclosporine A increases the uptake of AF18 by schistosomula in vitro which is dose-dependent, and decreases the parasite surface membrane fluidity.
ISSN:	1000-7423