The expression of HIF-1 in the early diabetic NOD mice

• Published in: Yanke xuebao Vol. 22; no. 2; p. 107
• Main Authors: Li, Cairui, Xu, Yan, Jiang, Deyong, Hong, Wei, Guo, Xiaojian, Wang, Ping, Li, Wengling
• Format: Journal Article
• Language: Chinese
• Published: China 01-06-2006
• Subjects: Animals; Diabetes Mellitus, Experimental - metabolism; Diabetes Mellitus, Experimental - pathology; Diabetic Retinopathy - metabolism; Female; Hypoxia-Inducible Factor 1, alpha Subunit - biosynthesis; Mice; Mice, Inbred NOD; Retina - metabolism; Retina - pathology
• ISSN: 1000-4432

To study the retinal expression of HIF-1 alpha in the diabetic NOD mice.
NOD mice were divided into normal control group and diabetes group randomly, sacrificed at 2, 4, 6, 8 or 12 weeks. Eyes were enucleated and retinas were isolated and used for western blot, or eyes were sectioned for immunohistochemical staining for HIF-1 alphal.
Compared to the normal control group, blood sugar of diabetes mice increased significantly but the weights decreased significantly (P < 0.01). Western blot of retinal lysates showed low levels of HIF-1 alpha at the 6th weeks that were markedly increased at the 12th weeks in the diabetes group. Immunohistochemistry showed increased staining for HIF-1 in the inner retina, but not in the outer retina.
The retinal HIF-1 alpha levels increasing showed that retinal ischemia happening in the early diabetes. HIF-1 alpha may participate in the secondary damage of retinal ischemia induced by early diabetes.