Structural and functional analyses of the Hfb1, Hmob3 and Hmob33 cDNAs as an example of human brain-specific gene studies

• Published in: Molekuliarnaia biologiia Vol. 37; no. 2; p. 315
• Main Authors: Dergunova, L V, Raevskaia, N M, Vladychenskaia, I P, Limborskaia, S A
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-03-2003
• Subjects: 3' Untranslated Regions; Adaptor Proteins, Vesicular Transport; Base Sequence; Basic Helix-Loop-Helix Transcription Factors; Brain - physiology; Cloning, Molecular; Conserved Sequence; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - genetics; Evolution, Molecular; Exons; Helix-Loop-Helix Motifs; Humans; Membrane Proteins; Microtubule-Associated Proteins - genetics; Molecular Sequence Data; Nerve Tissue Proteins - genetics; Organ Specificity; Phylogeny; RNA, Messenger; Sequence Analysis, DNA; Transcription Factors - chemistry; Transcription Factors - genetics; Transferases (Other Substituted Phosphate Groups)
• ISSN: 0026-8984

Brain-specific human genes were studied over the recent years in the Department of Molecular Bases of Human Genetics, Institute of Molecular Genetics. Clones Hfb1, Hmob3, and Hmob33 were selected from human brain cDNA libraries by differential screening. The clones were sequenced, mapped, and tested for expression in various human tissues. In vitro and in silico experiments identified Hfb1 as an earlier unknown complexin 2 gene (CPLX2) fragment, which codes for the large 3'-untranslated region of the CPLX2 mRNA. Hmob3 proved to correspond to an earlier unknown fragment of the large 3'-untranslated region of the human MAP1B mRNA. With Hfb1 and Hmob3, new terminal exons were revealed and exact structures established for CPLX2 and MAP1B. Hmob33 was identified as a fragment of the 3'-terminal exon of a new gene, MOB, which codes for a thus far unknown evolutionarily conserved transmembrane protein. The structure of the deduced protein product was analyzed.