Isolation, expression analysis of a chilling induced cDNA from rice root with differential display: an evidence role for caffeine-sensitive calcium signal

• Published in: Shengwu gongcheng xuebao Vol. 18; no. 4; p. 468
• Main Authors: Yin, Kui-De, Zhang, Xing-Mei, Liu, Shi-Qiang, Li, Le-Gong
• Format: Journal Article
• Language: Chinese
• Published: China 01-07-2002
• Subjects: Cold Temperature; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Mannitol - pharmacology; Oryza - drug effects; Oryza - genetics; Plant Roots - drug effects; Plant Roots - genetics; RNA, Messenger - drug effects; RNA, Messenger - genetics; RNA, Messenger - metabolism
• ISSN: 1000-3061

Chilling-sensitive rice varieties acquire chilling tolerance when their roots are exposed to water stress for short time. Caffeine-sensitive calcium signal was involved in this procedure. By using total RNA differential display, a chilling induced cDNA(ICT: induction of chilling treatment) was isolated from roots of chilling-sensitive rice variety. It was determined that it is a novel cDNA by homology searching. The transcript level of ict mRNA is up-regulated under chilling stress, it is decreased to low level when the samples were transferred to standard culture conditions. Pre-treated with mannitol for two hours is beneficial to inducing ICT level of expression. This chilling induction was inhibited by caffeine, suggesting that it may play a putative role in signal transduction of caffeine-sensitive calcium.