Angiotensin II stimulates cardiac L-type Ca(2+) current by a Ca(2+)- and protein kinase C-dependent mechanism

• Published in: American journal of physiology. Heart and circulatory physiology Vol. 280; no. 4; p. H1528
• Main Authors: Aiello, E A, Cingolani, H E
• Format: Journal Article
• Language: English
• Published: United States 01-04-2001
• Subjects: Alkaloids; Angiotensin II - pharmacology; Animals; Benzophenanthridines; Calcium - physiology; Calcium Channels, L-Type - drug effects; Calcium Channels, L-Type - physiology; Cats; Egtazic Acid - pharmacology; Enzyme Inhibitors - pharmacology; Heart - drug effects; Heart - physiology; In Vitro Techniques; Losartan - pharmacology; Membrane Potentials - drug effects; Membrane Potentials - physiology; Myocardium - enzymology; Naphthalenes - pharmacology; Patch-Clamp Techniques; Phenanthridines - pharmacology; Protein Kinase C - metabolism
• ISSN: 0363-6135
• DOI: 10.1152/ajpheart.2001.280.4.h1528

Angiotensin II (ANG II) evokes positive inotropic responses in various species. However, the effects of this peptide on L-type Ca(2+) currents (I(Ca)) are still controversial. We report in this study that the effects of ANG II on I(Ca) differ depending on the mode of patch-clamp technique used, standard whole cell (WC) or perforated patch (PP). No significant effects of ANG II (0.5 microM) were observed when WC in cells dialyzed with high EGTA was used. However, when the intracellular milieu was preserved using PP, ANG II induced a significant 77 +/- 6% increase in I(Ca) (-2.2 +/- 0.3 in control and -3.9 +/- 0.6 pA/pF in ANG II, n = 8, P < 0.05). When WC was used in cells dialyzed with low Ca(2+) buffer capacity (EGTA 0.1 mM), ANG II was able to induce an increase in I(Ca) (-3.5 +/- 0.3 in control vs. -4.8 +/- 0.4 pA/pF in ANG II, n = 13, P < 0.05). This increase was prevented when the cells were also dialyzed with the protein kinase C (PKC) inhibitor chelerythrine (50 microM) or calphostin C (1 microM). The above results allow us to conclude that strong intracellular Ca(2+) buffering prevents the physiological actions of ANG II on cardiac I(Ca), which are also dependent on activation of PKC.