Bioreduction of [alpha]-chloroacetophenone by whole cells of marine fungi

Bioreduction of [alpha]-chloroacetophenone by whole cells of marine fungi

The asymmetric reduction of 2-chloro-1-phenylethanone (1) by seven strains of marine fungi was evaluated and afforded (S)-(-)-2-chloro-1-phenylethanol with, in the best case, an enantiomeric excess of 50% and an isolated yield of 60%. The ability of marine fungi to catalyse the reduction was directl...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters Vol. 31; no. 10; pp. 1559 - 1563
Main Authors: Rocha, Lenilson C, Ferreira, Hercules V, Pimenta, Eli F, Berlinck, Roberto GS, Seleghim, Mirna HR, Javaroti, Darci CD, Sette, Lara D, Bonugli, Rafaella C, Porto, Andre LM
Format: Journal Article
Language:English
Published: Dordrecht Springer Nature B.V 01-10-2009
Subjects:
Asymmetry
Biotechnology
Cellular biology
Fungi
Glucose
Marine
Marine biology
Reduction
Sea water
Seawater
Strain
Substrates
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The asymmetric reduction of 2-chloro-1-phenylethanone (1) by seven strains of marine fungi was evaluated and afforded (S)-(-)-2-chloro-1-phenylethanol with, in the best case, an enantiomeric excess of 50% and an isolated yield of 60%. The ability of marine fungi to catalyse the reduction was directly dependent on growth in artificial sea water-based medium containing a high concentration of Cl super(-) (1.2M). When fungi were grown in the absence of artificial sea water, no reduction of 1 by whole cells was observed. The biocatalytic reduction of 1 was more efficient at neutral rather than acidic pH values and in the absence of glucose as co-substrate.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-009-0037-y