Prenatal determination of fetal RhD (rhesus positive) type by an amplification of DNA

Examination of RhD genotype (Rhesus D gene) from amniotic cells (or chorionic villi cells) by PCR amplification of DNA can be done at early stage of pregnancy. Due to some missing exons in the Rh partial D variant (e.g. DVI), it is necessary to use different PCR systems to get relevant results. The...

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Bibliographic Details
Published in:	Sbornik lekarsky Vol. 99; no. 1; p. 13
Main Authors:	Kvasnicka, J, Rypácková, B, Calda, P, Hájek, Z
Format:	Journal Article
Language:	English
Published:	Czech Republic 1998
Subjects:	Amniotic Fluid - chemistry Chorionic Villi - chemistry Female Fetal Blood - chemistry Genetic Carrier Screening Humans Infant, Newborn Male Polymerase Chain Reaction Pregnancy Prenatal Diagnosis Rh-Hr Blood-Group System - blood Rh-Hr Blood-Group System - genetics
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Summary:	Examination of RhD genotype (Rhesus D gene) from amniotic cells (or chorionic villi cells) by PCR amplification of DNA can be done at early stage of pregnancy. Due to some missing exons in the Rh partial D variant (e.g. DVI), it is necessary to use different PCR systems to get relevant results. The localization of primers in our three PCR systems is on the different exons (10, 7, and 4 + 5). The advantage of PCR technique is prenatal detection of RhD of fetus from nonerythrocytes suspension (e.g. from an amnion fluid cell sediment) in comparison to a "standard" haemagglutination serological technique which uses blood erythrocytes only. The possibility of this technique to distinguished the heterozygous (D/d) or homozygous (D/D) fathers can help the clinicians in decisions about the management of further prevention of the hemolytic disease of newborn.
ISSN:	0036-5327