Reevaluation of membrane binding of erythrocyte glutathione reductase (EC 1.6.4.2)

Data concerning membrane binding of glutathione reductase (GSSG-R) have been reexamined with respect to the following experimental conditions: 1. Haemolysis and membrane preparation; 2. influence of nonionic detergents (Triton X-100, Lubrol PX). Haemolysis and membrane preparations performed at phys...

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Bibliographic Details
Published in:	Acta biologica et medica Germanica Vol. 40; no. 9; p. 1129
Main Authors:	Földes-Papp, Z, Tsamaloukas, A G, Maretzki, D
Format:	Journal Article
Language:	English
Published:	Germany 1981
Subjects:	Adult Detergents - pharmacology Erythrocyte Membrane - enzymology Erythrocytes - enzymology Glutathione Reductase - blood Hemolysis Humans In Vitro Techniques Membrane Proteins - metabolism Osmolar Concentration
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Summary:	Data concerning membrane binding of glutathione reductase (GSSG-R) have been reexamined with respect to the following experimental conditions: 1. Haemolysis and membrane preparation; 2. influence of nonionic detergents (Triton X-100, Lubrol PX). Haemolysis and membrane preparations performed at physiological ionic strength, and reduced glutathione concentration do not give any evidence for binding of GSSG-R to the erythrocyte membrane. Under hypotonic conditions of ghost preparation about 2% of the total cellular GSSG-R activity are membrane bound, indicating weak enzyme association at low ionic strength only.
ISSN:	0001-5318