Identification by replicon-typing of indigenous plasmids of Burkholderia (Pseudomonas) solanacearum

Fifteen strains of Burkholderia (Pseudomonas) solanacearum were isolated, identified from different parts of India and screened for plasmid profile. They harboured 1-3 plasmids, but most of the isolates contained a single plasmid. Plasmids were classified on the basis of replicon typing by Southern...

Full description

Saved in:

Bibliographic Details
Published in:	Indian journal of experimental biology Vol. 35; no. 4; p. 408
Main Authors:	Elumalai, R P, Mahadevan, A
Format:	Journal Article
Language:	English
Published:	India 01-04-1997
Subjects:	Burkholderia - classification Burkholderia - genetics Burkholderia - isolation & purification India Molecular Probe Techniques Plasmids - genetics Replicon India
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Fifteen strains of Burkholderia (Pseudomonas) solanacearum were isolated, identified from different parts of India and screened for plasmid profile. They harboured 1-3 plasmids, but most of the isolates contained a single plasmid. Plasmids were classified on the basis of replicon typing by Southern hybridization using incompatible specific rep probes. Plasmids of 9 strains hybridized with repP probe, plasmids of 5 strains hybridized with repW probe and one plasmid hybridized with repQ probe. Fingerprint and cluster analyses of plasmids from 10 strains of B. solanacearum digested with Eco RI revealed that plasmids of race 3 strains clustered in one group and the plasmids of race 1 strains clustered in another group.
ISSN:	0019-5189