Quantitation of Bacillus anthracis by using of soybean agglutinin conjugates

Quantitation of Bacillus anthracis by using of soybean agglutinin conjugates

We examine the possibility of using the soybean agglutinin (SBA) marked by peroxidase (HRP), biotin, FITC, or gold in order to determine the number of Bacillus anthracis cells of vaccine strain STI. It was shown that the technique based on interaction between the lectin and microbial cell walls like...

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Bibliographic Details
Published in:Molekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 6; p. 27
Main Authors: Kalinin, N L, Shakhanina, K L, Kuljakina, M N
Format: Journal Article
Language:English
Published: Russia (Federation) 01-11-1993
Subjects:
Bacillus anthracis - isolation & purification
Biotin
Fluorescein-5-isothiocyanate
Glycine max
Gold Colloid
Horseradish Peroxidase
Immunoenzyme Techniques
Lectins
Plant Lectins
Soybean Proteins
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Summary:We examine the possibility of using the soybean agglutinin (SBA) marked by peroxidase (HRP), biotin, FITC, or gold in order to determine the number of Bacillus anthracis cells of vaccine strain STI. It was shown that the technique based on interaction between the lectin and microbial cell walls likely are not inferior in sensitivity to traditional ELISA variants. The sensitivities of methods were 10(4) cells/ml in the case of SBA-biotin, 10(5) cells/ml in the case of SBA-HRP, or 10(6) cells/ml in the cases of SBA-gold and SBA-FITC.
ISSN:0208-0613