Salt effects on the bacteriophage T7-II structure and activity changes

• Published in: Physiological chemistry and physics and medical NMR Vol. 19; no. 1; pp. 67 - 74
• Main Authors: Tóth, K, Csik, G, Rontó, G Y
• Format: Journal Article
• Language: English
• Published: United States 1987
• Subjects: Buffers; Calcium Chloride - pharmacology; Cations, Divalent; Cations, Monovalent; DNA, Viral - drug effects; Escherichia coli - drug effects; Escherichia coli - physiology; Escherichia coli - ultrastructure; Magnesium - pharmacology; Magnesium Chloride; Nucleic Acid Denaturation; Osmolar Concentration; T-Phages - drug effects; T-Phages - physiology; T-Phages - ultrastructure; Tromethamine - pharmacology
• ISSN: 0748-6642

Optically detected thermal stability and biological activity of phage T7 has been compared as the function of the ionic composition and strength of the buffers. The ionic strength range was studied between 20-140 mmol/1. In Tris buffer containing only monovalent ions the biological activity of the phages decreases abruptly below 50 mmol/1 ionic strength. Structural studies show a logarithmic dependence between the ionic strength and the intraphage DNA stability and no significant change in the thermal stability of the whole phage. Mg2+ and Ca2+ ions at low concentration (1 mmol/1) given into a Tris buffer of 20 mmol/1 original ionic strength highly stabilize the biological activity, which stabilization is also to be seen in the intraphage DNA and also in the whole phage thermal denaturation process.