Interleukin-8 mRNA Expression in Locally Advanced Colorectal Cancer Patients

• Published in: Current health sciences journal Vol. 43; no. 3; pp. 209 - 213
• Main Authors: Bărbălan, A, Streața, Ioana, Ivan, Elena Tatiana, Cherciu, Irina, Șurlin, V, Ioana, M, Săftoiu, A
• Format: Journal Article
• Language: English
• Published: Romania Medical University Publishing House Craiova 01-07-2017
• Subjects: Original Paper; advanced colorectal cancer; preoperative analysis; endoscopy; Interleukin-8 mRNA
• ISSN: 2067-0656; 2069-4032
• DOI: 10.12865/CHSJ.43.03.04

Interleukin-8 (IL-8) has been proven to promote progression of malignant tumours and control angiogenesis processes. We aim to determine and compare interleukin-8 (IL-8) gene expression level in colorectal tumors (CCR) and peritumoral samples obtained through endoscopic biopsy.
Total mRNA was obtained from both tumoral and peritumoral tissue samples collected from patients diagnosed with colorectal cancer. Through Quantitative Real Time PCR, IL-8 gene expression was assessed in both pathologic tissue and adjacent normal mucosa.
In our cohort, IL8 expression was higher in adjacent normal mucosa than in tumoral tissue, in all the samples. Further studies on larger groups are required to validate our results.