Expression of long non-coding RNA (lncRNA) H19 in immunodeficient mice induced with human colon cancer cells

• Published in: European review for medical and pharmacological sciences Vol. 20; no. 23; p. 4880
• Main Authors: Hu, Q, Wang, Y-B, Zeng, P, Yan, G-Q, Xin, L, Hu, X-Y
• Format: Journal Article
• Language: English
• Published: Italy 01-12-2016
• Subjects: Animals; Cell Line, Tumor; Colonic Neoplasms - genetics; Humans; Mice; MicroRNAs - genetics; RNA, Long Noncoding - genetics
• ISSN: 2284-0729

To study the expression of long non-coding RNA (lncRNA) H19 in immunodeficient mice induced with human colon cancer cells. Long non-coding RNAs (lncRNA) have a vital and regulatory role, in almost all cancers including colon cancer. In the present study, the prominent regulatory role of IncH19 RNA in immunodeficient mice induced with human colon cancer cells was identified. Interestingly, the lncH19 RNA was up-regulated in human colon cancer cells induced immunodeficient mice colon cancer samples.
The expression profile of the lnc H19 RNA in the colon cancer tissues were identified by RT-PCR and the further the data was confirmed and validated by Northern blot analysis.
Based on the data, it was identified that lnc H19 RNA was increased significantly in immunodeficient mice induced with human colon cancer cells, when compared with controls. The data was further confirmed and validated by Northern blot analysis.
The obtained data future potential application in colon cancer diagnosis. At the same time, lncH19 RNA may serve as a new target for anti-tumor therapy in colon cancer. Also, the obtained data is the important clue to find out the key roles of lncH19 RNA-miRNA functional network in colon cancer.