De-Epithelialized Connective Tissue Graft And The Reminiscent Epithelial Content After Harvested by the Harris' Technique: A Histological And Morphometrical Case Series

• Published in: Clinical advances in periodontics
• Main Authors: Maia, Vinicius Tadeu Gonçalves, Kahn, Sérgio, de Souza, Alex Balduino, Fernandes, Gustavo Vicentis de Oliveira
• Format: Journal Article
• Language: English
• Published: United States 01-09-2021
• Subjects: tissue grafts; epithelium; histology; plastic surgery; connective tissue; periodontics
• ISSN: 2163-0097
• DOI: 10.1002/cap.10151

The remaining epithelial layer existent in connective tissue graft (CTG) harvested from the hard palate, which underwent de-epithelization outside the oral cavity, can be histologically detected unless it is completely removed. Its presence may cause adverse esthetics results, affecting thus the color and texture of the receptor site, and an increased risk of presence of scar tissues after surgical procedures. The proposal of this study was to evaluate the CTG (histological and morphometrically) collected from the hard palate using the Harris' technique, removing the epithelial layer outside the mouth, assessing the remaining presence of epithelial tissue.
Fourteen patients (14CTGs) were included in the present case series study, therefore there were two dropouts. A small part of the tip of the graft was harvested and fixed in formalin solution for histological processing, staining, and then to be morphometrically analyzed. The epithelial tissue and CTGs were assessed by three calibrated and double-blinded professionals. All information was compiled, and the statistical analysis was performed. CTGs obtained had a width average of 1224.26 μm. There was no presence of any residual of the epithelium in three samples, whereas only one had the epithelium tissue covering the entire connective layer. Furthermore, seven samples (approximately 50%) had the presence of epithelium.
Within the limitation of this study, there was incomplete removal of the epithelial layer after harvesting the CTG using the Harris' technique (44.32%), most likely due to its histological persistency, suggesting to be inaccurate the clinical removal.