Impact of Hemoglobin Level in Ex Vivo Heart Perfusion on Donation After Circulatory Death Hearts: A Juvenile Porcine Experimental Model

• Published in: Transplantation Vol. 108; no. 9; p. 1922
• Main Authors: Kobayashi, Yasuyuki, Li, Jing, Parker, Marlee, Wang, Jian, Nagy, Anita, Fan, Chun-Po Steve, Runeckles, Kyle, Okumura, Michiru, Kadowaki, Sachiko, Honjo, Osami
• Format: Journal Article
• Language: English
• Published: United States 01-09-2024
• Subjects: Animals; Heart Transplantation; Hemoglobins - analysis; Hemoglobins - metabolism; Models, Animal; Myocardium - metabolism; Myocardium - pathology; Organ Preservation - methods; Oxygen Consumption; Perfusion - methods; Sus scrofa; Swine
• ISSN: 1534-6080
• DOI: 10.1097/TP.0000000000004954

Ex vivo heart perfusion (EVHP) of donation after circulatory death (DCD) hearts has become an effective strategy in adults; however, the small circulating volume in pediatrics poses the challenge of a low-hemoglobin (Hb) perfusate. We aimed to determine the impact of perfusate Hb levels during EVHP on DCD hearts using a juvenile porcine model.
Sixteen DCD piglet hearts (11-14 kg) were reperfused for 4 h in unloaded mode followed by working mode. Metabolism, cardiac function, and cell damage were compared between the low-Hb (Hb, 5.0-5.9 g/dL; n = 8) and control (Hb, 7.5-8.4 g/dL; n = 8) groups. Between-group differences were evaluated using 2-sample t -tests or Fisher's Exact tests.
During unloaded mode, the low-Hb group showed lower myocardial oxygen consumption ( P  < 0.001), a higher arterial lactate level ( P  = 0.001), and worse systolic ventricular function ( P  < 0.001). During working mode, the low-Hb group had a lower cardiac output (mean, 71% versus 106% of normal cardiac output, P  = 0.010) and a higher arterial lactate level ( P  = 0.031). Adjusted cardiac troponin-I ( P  = 0.112) did not differ between the groups. Morphological myocyte injury in the left ventricle was more severe in the low-Hb group ( P  = 0.028).
Low-Hb perfusate with inadequate oxygen delivery induced anaerobic metabolism, resulting in suboptimal DCD heart recovery and declined cardiac function. Arranging an optimal perfusate is crucial to organ protection, and further endeavors to refine the priming volume of EVHP or the transfusion strategy are required.