An aptamer and hydroxyapatite-silver-zinc oxide–based novel electrochemical sensor for ultrasensitive H. pylori detection

• Published in: Mikrochimica acta (1966) Vol. 191; no. 12; p. 751
• Main Authors: Rafique, Saima, Akram, Rizwan, Nasir, Rubina, Naz, Nafeesa, Shafique, Adnan, Bashir, Shazia, Haq, Zeenat
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-12-2024; Springer Nature B.V
• Subjects: Analytical Chemistry; Biosensors; Characterization and Evaluation of Materials; Chemical sensors; Chemistry; Chemistry and Materials Science; Composite materials; Conducting polymers; Dynamic range; Electrochemical impedance spectroscopy; Electrodes; Functional groups; Glassy carbon; Hydroxyapatite; Microengineering; Nanochemistry; Nanotechnology; Polythiophene; Reproducibility; Silver; Square waves; Stability; Voltammetry; Zinc oxide; Zinc oxides; Modified glassy carbon electrode; Square wave voltammetry; Hydroxyapatite; Polythiophene; Aptasensor; Hap-Ag-ZnO composite
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-024-06834-y

Electrochemical biosensors offer a cost-effective way to identify
Helicobacter pylori
(
H. pylori
) (Hsp 60), responsible for stomach infections. The conductivity and surface area of the electrode were enhanced using Hap-Ag-ZnO composites on glassy carbon electrode (GCE) along with polythiophene (PP), as a conductive polymer to improve proficiency. The Hap-Ag-ZnO-PP composites have multiple functional group sites that facilitate aptamer immobilization on the GCE. This increases the sensitivity, stability, and dynamic range of the developed aptasensor. Electrochemical impedance spectroscopy, cyclic voltammetry, and square wave voltammetry were employed to examine the performance of the aptasensor. A linear behavior was observed for the modified electrode against the concentration of Hsp 60, showing a detection limit of 0.429 nM and a wide dynamic range of 0.05–300 nM. Moreover, the Hap-Ag-ZnO-PP-based aptasensor exhibits exceptional reproducibility, repeatability, fast response time (20 min), good selectivity, and excellent stability (electrochemical and storage). Therefore, the prepared sensitive aptasensor provides a promising platform for clinical diagnostics and
H. pylori
detection in real-world applications.
Graphical Abstract