Serum Levels of TNF- alpha , TNF- alpha RI, TNF- alpha RII and IL-12 in Treated Rheumatoid Arthritis Patients

• Published in: Iranian journal of immunology Vol. 6; no. 3; pp. 147 - 153
• Main Authors: Ebrahimi, A A, Noshad, H, Sadreddini, S, Hejazi, MS, Sadigh, Y M, Eshraghi, Y, Ghojazadeh, M
• Format: Journal Article
• Language: English
• Published: 01-09-2009
• ISSN: 1735-1383

Background: Rheumatoid arthritis (RA) is a chronic multisystem autoimmune disease common in all races and ethnics. Cytokines and cytokines receptors play an important role in RA pathogenesis and clinical presentation. Objective: To investigate the serum levels of TNF- alpha , TNF- alpha RI, TNF- alpha RII and IL-12 in RA patients and healthy control group. Methods: In this study 43 patients fulfilling the revised criteria of American College of Rheumatology (ACR) for RA and 13 healthy cases as a control group were selected for TNF- alpha , TNF- alpha RI, TNF- alpha RII and IL-12 serum level analysis. The patients' age was 42.2 plus or minus 22 and the age of healthy group was 40.1 plus or minus 19.2 years (p=0.1). The patients had an active disease with at least six swollen and ten tender joints. Minimum ESR was 28 mm at first hours of the morning. Early morning stiffness in patients lasted longer than 45 minutes. Results: Our study showed that IL-12 serum level of the patients (91.69 plus or minus 43.07 pg/ml) and control (61.79 plus or minus 40.08 pg/ml) group was significantly different (p<0.001). The serum level of TNF- alpha RI was 2.36 plus or minus 0.77 ng/ml in the patient and 1.73 plus or minus 0.37 ng/ml in the control group (p<0.01). TNF- alpha RII serum concentration in patients was 8.89 plus or minus 2.3 ng/ml, while that of control group was 7.06 plus or minus 1.30 ng/ml (p=0.03). The serum level of TNF- alpha in patients was 32.90 plus or minus 19.27 pg/ml and that of the control group was 24.27 plus or minus 8.28 pg/ml (p=0.08) with no significant difference between the two. Conclusions: It is concluded that IL-12, TNF- alpha RI and TNF- alpha RII serum concentrations are more important and better predictive factors than TNF- alpha in RA course and in the active forms of the disease.