Protective effects of silymarin on fumonisin B₁-induced hepatotoxicity in mice

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 15; no. 1; pp. 51 - 60
• Main Authors: Sozmen, Mahmut, Devrim, Alparslan Kadir, Tunca, Recai, Bayezit, Murat, Dag, Serpil, Essiz, Dinc
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 2014; The Korean Society of Veterinary Science
• Subjects: Animals; Antioxidants - pharmacology; Apoptosis - drug effects; Cell Proliferation - drug effects; Female; Fibroblast Growth Factor 2 - genetics; Fibroblast Growth Factor 2 - metabolism; Fumonisins - toxicity; Gene Expression Regulation - drug effects; Hepatocytes - drug effects; Ki-67 Antigen - metabolism; Liver - drug effects; Mice; Mice, Inbred BALB C; Mycotoxins - toxicity; Neovascularization, Physiologic - drug effects; Original; Proliferating Cell Nuclear Antigen - metabolism; Silymarin - pharmacology; Tumor Necrosis Factor-alpha - metabolism; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; 수의학; fibroblast growth factor-2; caspase-8; silymarin; galectin-3; fumonisin B1
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2014.15.1.51

The present study was conducted to investigate the effect of silymarin on experimental liver toxication induced by Fumonisin B₁1 (FB₁) in BALB/c mice. The mice were divided into six groups (n = 15). Group 1 served as the control. Group 2 was the silymarin control (100 mg/kg by gavage). Groups 3 and 4 were treated with FB₁ (Group 3, 1.5 mg/kg FB₁, intraperitoneally; and Group 4, 4.5 mg/kg FB₁). Group 5 received FB₁ (1.5 mg/kg) and silymarin (100 mg/kg), and Group 6 was given a higher dose of FB₁ (4.5 mg/kg FB₁) with silymarin (100 mg/kg). Silymarin treatment significantly decreased (p < 0.0001) the apoptotic rate. FB1 administration significantly increased (p < 0.0001) proliferating cell nuclear antigen and Ki-67 expression. Furthermore, FB1 elevated the levels of caspase-8 and tumor necrosis factor-alpha mediators while silymarin significantly reduced (p < 0.0001) the expression of these factors. Vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) expressions were significantly elevated in Group 4 (p < 0.0001). Silymarin administration alleviated increased VEGF and FGF-2 expression levels (p < 0.0001). In conclusion, silymarin ameliorated toxic liver damage caused by FB₁ in BALB/c mice.