Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family

Amino-acid sequences derived from complementary DNAs encoding the alpha- and beta-subunits of the GABA/benzodiazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the...

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Bibliographic Details
Published in:	Nature (London) Vol. 328; no. 6127; pp. 221 - 227
Main Authors:	Schofield, Peter R, Darlison, Mark G, Fujita, Norihisa, Burt, David R, Stephenson, F. Anne, Rodriguez, Henry, Rhee, Lucy M, Ramachandran, J, Reale, Vincenzina, Glencorse, Thora A, Seeburg, Peter H, Barnard, Eric A
Format:	Journal Article
Language:	English
Published:	England Nature Publishing Group 16-07-1987
Subjects:	Animals Cattle Cloning, Molecular Deoxyribonucleic acid DNA DNA - genetics gamma-Aminobutyric Acid - physiology Gene Expression Regulation Genetics Membrane Proteins - genetics Multigene Family Oocytes - physiology Protein Conformation Receptors, GABA-A - genetics Receptors, GABA-A - physiology Receptors, Nicotinic - genetics
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Summary:	Amino-acid sequences derived from complementary DNAs encoding the alpha- and beta-subunits of the GABA/benzodiazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the in vitro-generated alpha-subunit and beta-subunit RNAs in Xenopus oocytes produces a functional receptor and ion channel with the pharmacological properties characteristic of the GABAA receptor.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0028-0836 1476-4687
DOI:	10.1038/328221a0