Identification of internal autoproteolytic cleavage sites within the prosegments of recombinant procathepsin B and procathepsin S. Contribution of a plausible unimolecular autoproteolytic event for the processing of zymogens belonging to the papain family

• Published in: The Journal of biological chemistry Vol. 276; no. 11; pp. 8118 - 8124
• Main Authors: Quraishi, O, Storer, A C
• Format: Journal Article
• Language: English
• Published: United States 16-03-2001
• Subjects: Amino Acid Sequence; Binding Sites; Catalysis; Cathepsin B - chemistry; Cathepsin B - metabolism; Cathepsins - chemistry; Cathepsins - metabolism; Enzyme Precursors - chemistry; Enzyme Precursors - metabolism; Humans; Hydrogen-Ion Concentration; Molecular Sequence Data; Papain - metabolism; Protein Conformation; Recombinant Proteins - metabolism
• ISSN: 0021-9258
• DOI: 10.1074/jbc.M005851200

The steps involved in the maturation of proenzymes belonging to the papain family of cysteine proteases have been difficult to characterize. Intermolecular processing at or near the pro/mature junction, due either to the catalytic activity of active enzyme or to exogeneous proteases, has been well documented for this family of proenzymes. In addition, kinetic studies are suggestive of a slow unimolecular mechanism of autoactivation which is independent of proenzyme concentration. However, inspection of the recently determined x-ray crystal structures does not support this evidence. This is due primarily to the extensive distances between the catalytic thiolate-imidazolium ion pair and the putative site of proteolysis near the pro/mature junction required to form mature protein. Furthermore, the prosegments for this family of precursors have been shown to bind through the substrate binding clefts in a direction opposite to that expected for natural substrates. We report, using cystatin C- and N-terminal sequencing, the identification of autoproteolytic intermediates of processing in vitro for purified recombinant procathepsin B and procathepsin S. Inspection of the x-ray crystal structures reported to date indicates that these reactions occur within a segment of the proregion which binds through the substrate binding clefts of the enzymes, thus suggesting that these reactions are occurring as unimolecular processes.